Spectrophotometric Determination of Cefixime Following Simple Diazotization and Coupling with ?-Naphthol

  • Samar A. Darweesh

Abstract

Cefixime (CFX) was treated with sodium nitrite and hydrochloric acid for diazotization reaction followed by coupling with ?-Naphthol in alkaline medium to form, a yellow colored azo dye compound which exhibits maximum absorption (?max) at 412 nm where the concentration of (CFX) was determined spectrophotometrically. The optimum reaction conditions and other analytical parameters were evaluated. Beer’s law was obeyed in the concentration range of (1-20) ?g.mL-1 with a molar absorptivity of 34870.5 L.mol-1.cm-1. The limit of detection was found to be 0.1090 ?g.mL-1 and the Sandell's sensitivity value was 0.0130 ?g.cm-2. The proposed method could be successfully applied to the determination of (CFX) in pharmaceutical formulations.

Published
Dec 25, 2017
How to Cite
A. DARWEESH, Samar. Spectrophotometric Determination of Cefixime Following Simple Diazotization and Coupling with ?-Naphthol. Iraqi Journal of Pharmaceutical Sciences ( P-ISSN: 1683 - 3597 , E-ISSN : 2521 - 3512), [S.l.], p. 1-6, dec. 2017. ISSN 1683-3597. Available at: <http://www.bijps.com/index.php/bijps/article/view/726>. Date accessed: 15 dec. 2018.